gfap - cre ( Search Results


glial fibrillary acidic protein gfap promoter  (Addgene inc)
93
Addgene inc glial fibrillary acidic protein gfap promoter
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Glial Fibrillary Acidic Protein Gfap Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glial fibrillary acidic protein gfap promoter/product/Addgene inc
Average 93 stars, based on 1 article reviews
glial fibrillary acidic protein gfap promoter - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
gfapcre vector  (Addgene inc)
90
Addgene inc gfapcre vector
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfapcre Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfapcre vector/product/Addgene inc
Average 90 stars, based on 1 article reviews
gfapcre vector - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gfap-cre 012886  (Jackson Laboratory)
90
Jackson Laboratory gfap-cre 012886
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfap Cre 012886, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfap-cre 012886/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
gfap-cre 012886 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gfap-cre breeders  (Jackson Laboratory)
90
Jackson Laboratory gfap-cre breeders
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfap Cre Breeders, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfap-cre breeders/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
gfap-cre breeders - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
fvb-tg(gfap-cre)25mes/j mice  (Jackson Laboratory)
90
Jackson Laboratory fvb-tg(gfap-cre)25mes/j mice
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Fvb Tg(Gfap Cre)25mes/J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fvb-tg(gfap-cre)25mes/j mice/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
fvb-tg(gfap-cre)25mes/j mice - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gfap::cre transgenic mice cre77.6  (Jackson Laboratory)
90
Jackson Laboratory gfap::cre transgenic mice cre77.6
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfap/Cre Transgenic Mice Cre77.6, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfap::cre transgenic mice cre77.6/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
gfap::cre transgenic mice cre77.6 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gfap- tk mice  (Shanghai Model Organisms Center)
90
Shanghai Model Organisms Center gfap- tk mice
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfap Tk Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfap- tk mice/product/Shanghai Model Organisms Center
Average 90 stars, based on 1 article reviews
gfap- tk mice - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
mouse gfap-specific cre transgenic mice gfap-cre mice; stock no. 024098  (Jackson Laboratory)
90
Jackson Laboratory mouse gfap-specific cre transgenic mice gfap-cre mice; stock no. 024098
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Mouse Gfap Specific Cre Transgenic Mice Gfap Cre Mice; Stock No. 024098, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse gfap-specific cre transgenic mice gfap-cre mice; stock no. 024098/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
mouse gfap-specific cre transgenic mice gfap-cre mice; stock no. 024098 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gfap cre line 004600  (Jackson Laboratory)
90
Jackson Laboratory gfap cre line 004600
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Gfap Cre Line 004600, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfap cre line 004600/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
gfap cre line 004600 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
cross-breeds of gfap-cre  (Jackson Laboratory)
90
Jackson Laboratory cross-breeds of gfap-cre
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Cross Breeds Of Gfap Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cross-breeds of gfap-cre/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
cross-breeds of gfap-cre - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
mice heterozygous for gfap-cre  (Jackson Laboratory)
90
Jackson Laboratory mice heterozygous for gfap-cre
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
Mice Heterozygous For Gfap Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mice heterozygous for gfap-cre/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
mice heterozygous for gfap-cre - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
n1icd  (StemCells Inc)
90
StemCells Inc n1icd
a , Systemic delivery of two different <t>GFAP-based</t> promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.
N1icd, supplied by StemCells Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n1icd/product/StemCells Inc
Average 90 stars, based on 1 article reviews
n1icd - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


a , Systemic delivery of two different GFAP-based promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.

Journal: bioRxiv

Article Title: A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences

doi: 10.1101/2023.02.21.529451

Figure Lengend Snippet: a , Systemic delivery of two different GFAP-based promoters (GFAP and GfaABC1D), co-injected with PHP.eB::CAG-flex-GFP, yield high levels of transduction of non-astrocytic cells. Sox9, astrocytes; NeuN, neurons. b , Characterization of PHP.eB::GfaABC1D-Cre delivery in transgenic (Tg) Ai14-tdTomato mice: while the majority of tdTomato + cells are astrocytes, there are high numbers of transduced neurons (NeuN + ) and low numbers of endothelial cells (CD31 + ; yellow arrow). c , Schematic diagram of miRNA targeting approach. d , Changes in astrocytic selectivity by the addition of multiple copies of single miR targeting sequences; compared to GfaABC1D-Cre via unpaired t-test. miR124T.4x: 4 copies, ****p<0.0001. miR137T.2x: two copies, *p=0.0251. miR329T.2x: two copies, p=0.2909. miR369T.2x: two copies, p=0.3187. miR431T.2x: two copies, *p=0.0398. e , Schematic diagram of final AAV::GfaABC1D-Cre-4×6T plasmid. f , Enhanced astrocyte specificity with PHP.eB::GfaABC1D-Cre-4×6T, using a PHP.eB::CAG-flex-GFP reporter, a transgenic Ai14-tdTomato reporter, or a PHP.eB::CAG-flex-smV5-4×6T reporter. Using a transgenic mouse line or including the 4×6T cassette on the flex reporter increases astrocyte specificity (one-way ANOVA, Tukey’s multiple comparisons test; flexGFP vs Ai14, *p=0.0249; flexGFP vs flexV5-4×6T, *p=0.0259). All data presented as mean ± SEM; n = 3-4 mice per condition. Scale bars: 40μm.

Article Snippet: To assess the astrocytic specificity of Cre viral delivery using current vectors for systemic delivery, we administered PHP.eB viruses carrying Cre under a glial fibrillary acidic protein (GFAP) promoter (PHP.eB::GFAP-Cre; Addgene 105550); GFAP is an astrocyte-specific protein and elements of the promoter have been widely used to target astrocytes .

Techniques: Injection, Transduction, Transgenic Assay, Plasmid Preparation

a , Immunohistochemical analysis of RiboTag + cells (hemagglutinin HA + ribosomal tag), colocalized with astrocytic marker Aldh1l1 and astrocytic reactivity marker GFAP. Astrocytic specificity is high in Aldh1l1-CreERT2 transgenic mice and systemic delivery of PHP.eB::GfaABC1D-Cre-4×6T with no overt evidence of astrocyte reactivity (GFAP % coverage). Specificity remains high with intracortical delivery of PHP.eB::GfaABC1D-Cre-4×6T and decreases with intracortical delivery of AAV2/5::GfaABC1D-Cre; this route of viral delivery shows some evidence of astrocyte reactivity by GFAP immunoreactivity. Scale bars: 50μm. mean ± SEM; n = 4 mice per brain region. Astrocyte specificity, HA + Aldh1l1 + /HA + : one-way ANOVA, Holm-Sidak’s multiple comparisons test, Aldh1l1-CreERT2 vs AAV2/5::GfaABC1D-Cre ****p<0.0001. GFAP % area coverage: one-way ANOVA, Holm-Sidak’s multiple comparisons test; Aldh1l1-CreERT2 vs PHP.eB::GfaABC1D-Cre-4×6T *p=0.0431; Aldh1l1-CreERT2 vs AAV2/5::GfaABC1D-Cre *p=0.0481. b , Relative levels of enrichment and de-enrichment of canonical genes for astrocytes, neurons, oligodendrocytes, microglia, and endothelial cells in IP vs input samples. c , Differentially expressed genes in IP samples: different viral cohorts vs Aldh1l1-CreERT2 IP samples, and systemic vs cortical 4×6T samples. FDR < 0.05, average FPKM across all IP samples > 1. d , Cell-type specificity of differentially expressed genes in IP samples: genes in (c) that are represented in the top 1000 specific genes for astrocytes, endothelial cells, microglia, neurons, or oligodendrocytes. e , Venn diagram showing the overlap of which neuron-specific genes from (d) are upregulated in different viral cohort IP samples vs Aldh1l1-CreERT2 samples. f , Top ten most significantly upregulated and downregulated Gene Ontology gene sets (FDR < 0.05) in ranked IP transcriptomes of each of the viral cohorts, calculated with Gene Set Enrichment Analysis; some gene sets overlapped, particularly for 4×6T cohorts, and only 7 gene sets passed FDR < 0.05 for intracortical 4×6T. In cases where the FDR was 0, scores were reassigned as 0.0001 to visualize the relative -logFDR. g , Numbers of genes potentially regulated by miRNAs that comprise the 4×6T cassette (5409 total) that show evidence of 4×6T-specific regulation in IP samples (FDR < 0.05 in either 4×6T cohort vs Aldh1l1-CreERT2 cohort; not differentially regulated in AAV2/5 cohort vs Aldh1l1-CreERT2 cohort). Note: none of the 5409 genes show evidence of 4×6T-specific regulation in input samples. n = 4 mice per cohort.

Journal: bioRxiv

Article Title: A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences

doi: 10.1101/2023.02.21.529451

Figure Lengend Snippet: a , Immunohistochemical analysis of RiboTag + cells (hemagglutinin HA + ribosomal tag), colocalized with astrocytic marker Aldh1l1 and astrocytic reactivity marker GFAP. Astrocytic specificity is high in Aldh1l1-CreERT2 transgenic mice and systemic delivery of PHP.eB::GfaABC1D-Cre-4×6T with no overt evidence of astrocyte reactivity (GFAP % coverage). Specificity remains high with intracortical delivery of PHP.eB::GfaABC1D-Cre-4×6T and decreases with intracortical delivery of AAV2/5::GfaABC1D-Cre; this route of viral delivery shows some evidence of astrocyte reactivity by GFAP immunoreactivity. Scale bars: 50μm. mean ± SEM; n = 4 mice per brain region. Astrocyte specificity, HA + Aldh1l1 + /HA + : one-way ANOVA, Holm-Sidak’s multiple comparisons test, Aldh1l1-CreERT2 vs AAV2/5::GfaABC1D-Cre ****p<0.0001. GFAP % area coverage: one-way ANOVA, Holm-Sidak’s multiple comparisons test; Aldh1l1-CreERT2 vs PHP.eB::GfaABC1D-Cre-4×6T *p=0.0431; Aldh1l1-CreERT2 vs AAV2/5::GfaABC1D-Cre *p=0.0481. b , Relative levels of enrichment and de-enrichment of canonical genes for astrocytes, neurons, oligodendrocytes, microglia, and endothelial cells in IP vs input samples. c , Differentially expressed genes in IP samples: different viral cohorts vs Aldh1l1-CreERT2 IP samples, and systemic vs cortical 4×6T samples. FDR < 0.05, average FPKM across all IP samples > 1. d , Cell-type specificity of differentially expressed genes in IP samples: genes in (c) that are represented in the top 1000 specific genes for astrocytes, endothelial cells, microglia, neurons, or oligodendrocytes. e , Venn diagram showing the overlap of which neuron-specific genes from (d) are upregulated in different viral cohort IP samples vs Aldh1l1-CreERT2 samples. f , Top ten most significantly upregulated and downregulated Gene Ontology gene sets (FDR < 0.05) in ranked IP transcriptomes of each of the viral cohorts, calculated with Gene Set Enrichment Analysis; some gene sets overlapped, particularly for 4×6T cohorts, and only 7 gene sets passed FDR < 0.05 for intracortical 4×6T. In cases where the FDR was 0, scores were reassigned as 0.0001 to visualize the relative -logFDR. g , Numbers of genes potentially regulated by miRNAs that comprise the 4×6T cassette (5409 total) that show evidence of 4×6T-specific regulation in IP samples (FDR < 0.05 in either 4×6T cohort vs Aldh1l1-CreERT2 cohort; not differentially regulated in AAV2/5 cohort vs Aldh1l1-CreERT2 cohort). Note: none of the 5409 genes show evidence of 4×6T-specific regulation in input samples. n = 4 mice per cohort.

Article Snippet: To assess the astrocytic specificity of Cre viral delivery using current vectors for systemic delivery, we administered PHP.eB viruses carrying Cre under a glial fibrillary acidic protein (GFAP) promoter (PHP.eB::GFAP-Cre; Addgene 105550); GFAP is an astrocyte-specific protein and elements of the promoter have been widely used to target astrocytes .

Techniques: Immunohistochemical staining, Marker, Transgenic Assay